ABSTRACT
<p><b>OBJECTIVE</b>To study the toxicity of water extracts from the fruits of Evodia Fructus in different producing areas.</p><p><b>METHOD</b>Compare the toxicity of the extracts from different Evodia Fructus on mice by the methods of acute and subacute toxicity test. The mice were given the extracts for 1 d to test the maximal tolerance dose (MTD) or maximal dose and observe the acute toxic symptoms; The mice were given the extracts for 15 d and then detected the level of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG). The liver index was calculated, and the liver histological changes were investigated.</p><p><b>RESULT</b>The MTD of water extracts from the fruits of Evodia Fructus is 62, 44.8, 35.84 g x kg(-1); the MTD of Evodia Fructus is 56, 44. 8, 35.84 g x kg(-1); the maximal dose of Evodia Fructus is 60, 54, 45 g x kg(-1). The toxic symptoms of the mice which had been given the nine samples were almost consistent. Compared with the control group in subacute toxicity test, the level of serum ALT and the liver index were all increased. The liver histological were changed.</p><p><b>CONCLUSION</b>When water extracts from the fruits of Evodia Fructus are given to mice one or more times. It may be toxic and induce liver damage. There is no significant correlation between the toxicity and Evodia orgins, while the toxicity seems to be more closely related to the producing area.</p>
Subject(s)
Animals , Female , Male , Mice , Alanine Transaminase , Blood , Aspartate Aminotransferases , Blood , China , Drugs, Chinese Herbal , Metabolism , Toxicity , Evodia , Chemistry , Fruit , Chemistry , Liver , Metabolism , Triglycerides , BloodABSTRACT
<p><b>OBJECTIVE</b>To determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.</p><p><b>METHOD</b>By HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.</p><p><b>RESULT</b>Carboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).</p><p><b>CONCLUSION</b>This method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.</p>
Subject(s)
Asteraceae , Chemistry , Atractyloside , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Fruit , ChemistryABSTRACT
A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
Subject(s)
Chromatography, High Pressure Liquid , Methods , Evodia , Chemistry , Indole Alkaloids , Limonins , Plant Extracts , QuinazolinesABSTRACT
<p><b>OBJECTIVE</b>To study the protective effect of Shaoganduogan (SGDG) on serum transaminase, liver pathology and hepatocyte mitochondria in rat with subacute liver injury induced by carbon tetrachloride.</p><p><b>METHOD</b>Subacute liver injury of rats were induced by carbon tetrachloride, and cured by different doses of SGDG through intragastric administration. The activity of serum ALT, AST, liver pathology and ultrastructure, activity of ATPase, SOD and content of MDA of hepatocyte mitochondria were observed.</p><p><b>RESULT</b>SGDG can remarkably reduce the transaminase, alleviate the degeneration and necrosis of liver cells ,enhance activity of Na+ -K+ ATPase, Ca2+ ATPase, SOD, reduce content of MDA of mitochondria, alleviate ultrastructure change of mitochondria, reduce section area, perimeter equivalent diameter and average optical density perimeter of liver cells.</p><p><b>CONCLUSION</b>SGDG has obvious effect of liver protection, the mechanisms are related with alleviating mitochondria injury.</p>
Subject(s)
Animals , Male , Rats , Carbon Tetrachloride , Chemical and Drug Induced Liver Injury , Pathology , Drugs, Chinese Herbal , Pharmacology , Hepatocytes , Pathology , Malondialdehyde , Metabolism , Mitochondria , Metabolism , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To study the transportation of Xiaochaihu Tang in Caco-2 cell model.</p><p><b>METHOD</b>The safety concentration of Xiaochaihu Tang in Caco-2 cells was determined by MTT assay. Then the Caco-2 cell model was used to investigate the bi-directional transportation of Xiaochaihu Tang. The multicomponents of Xiaochaihu Tang and the influence of time were measured by high performance liquid chromatography (HPLC).</p><p><b>RESULT</b>The P(app) values of wogonoside and wogonin were (1.23 +/- 0.09) x 10(-6), (1.07 +/- 0.89) x 10(-5) cm x s(-1) from the AP side to BL side, and (2.12 +/- 0.19) x 10(-6) and (7.12 +/- 1.02) x 10(-6) cm x s(-1) from the BL side to AP side, respectively. The P(appAP --> BL)/P(app BL --> AP) ratio of wogonoside and wogonin were 0.58 and 1.49, respectively. Baicalin, baicalein and glycyrrhizic acid could not permeate the Caco-2 cell model.</p><p><b>CONCLUSION</b>The transportation of wogonoside and wogonin in Caco-2 cell model may be a passive transportation.</p>
Subject(s)
Humans , Biological Transport, Active , Caco-2 Cells , Metabolism , Cell Line, Tumor , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Pharmacokinetics , Flavanones , Metabolism , Flavonoids , Metabolism , Glucosides , Metabolism , Glycyrrhizic Acid , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.</p><p><b>METHOD</b>HPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.</p><p><b>RESULT</b>Baifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.</p><p><b>CONCLUSION</b>This method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.</p>
Subject(s)
Aconitum , Chemistry , Alkaloids , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Reference Standards , Medicine, Chinese Traditional , Reference Standards , Quality ControlABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC-PDA method for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang.</p><p><b>METHOD</b>A Symmetry Shield RP18 (4.6 mm x 250 mm, 5.0 microm) was used with a mobile phase of acetonitrile-0.01% H3PO4 in gradient elution. The detection wavelength was 251 nm,the flow rate was 0.45 mL x min(-1) and the column temperature was maintained at 30 degrees C.</p><p><b>RESULT</b>The accuracy, precision, sensitivity, specificity and linearity of this method met the requirements. The contents of the five effective fractions were determined simultaneously.</p><p><b>CONCLUSION</b>The method is rapid,simple and accurate and it can be suitable for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Flavanones , Flavonoids , Glucosides , Glycyrrhizic Acid , Indicators and Reagents , Medicine, Chinese Traditional , Rehmannia , Chemistry , Spectrophotometry, Ultraviolet , Methods , Technology, PharmaceuticalABSTRACT
Objective: To determine tannins in Ershiwuweidatang Capsules (Flos Carthami, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, etc.). Methods:Tannins were determined by casein method.Results: The contents of tannins in Ershiwuweidatang Capsules were 0.96%, 0.90%, and 0.85%, respectively. The gallic acid calibration curves were linear at the ranges of 0.8 ～4.0 ?g?mL -1 ( r = 0.999 ). The average recovery of tannic acid was 96.2% with RSD =1.4%( n =5).Conclusion: The method was simple and reliable.
ABSTRACT
Objective: To establish the determination method of ester alkaloids in Shiwuweirupeng Capsules. Methods:The chromatographic method was carried out on Aichrom TM C 18 column using CH 3OH 0.05 mol?L -1 KH 2PO 4 CH 3COOH-(CH 3) 2CHOH(67∶173∶4∶4) as a mobile phase. The detection wavelength was set at 230nm. The flow rate was 1mL?min -1 . The column temperature was 35?C. Results: The benzoic acid calibration curves were linear at the ranges of 0.0152～0.076?g( r =0.9998). The average recoveries of aconitine was 93.3% with RSD =1.9%.Conclusion: This method is simple, reliable and provides a reference standard for the quality control of Shiwuweirupeng Capsules.
ABSTRACT
AIM:To prepare Wurenchun Dispersible Tablet(Fructus schisandrae Chinensis)and investigate the dissolution of it.METHODS:The optimal formulation of Wurenchun Dispersible Tablets was established by the single factor experiments.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was determined by HPLC.RESULTS:Wurenchun Dispersible Tablets prepared by the mixture of 50 g extraction and 125 g CaHPO_4 plus excipent(MCC∶CMS-Na 3∶1)could disintegrate and disperse well within 3 min in(20?1)℃ water.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was higher than 80% in 15 min.CONCLUSION:Wurenchun Dispersible Tablets were stable,dissolved fast and completely.The preparation could promote the dissolution of total lignans.